acridine orange propidium iodide (Fisher Scientific)
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Acridine Orange Propidium Iodide, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/acridine+orange+propidium+iodide/acridine+orange/pm42143044-290-67-71
Average 86 stars, based on 1 article reviews
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Suspension:Article Title: Developing a durable, memory-driven, CspZ-targeting Lyme disease vaccine by rationale adjuvant selection. Article Snippet: Spleens were rinsed and homogenized in sterile PBS buffer, using a AR TI CL E IN P RE SS gentleMACS Dissociator (Miltenyi Biotech, Charlestown, MA) and treated with ACK lysis buffer (Lonza, Portsmouth, NH) to remove red blood cells. .. The suspension was then diluted 5-fold with complete Roswell Park Memorial Institute (RPMI) 1640 medium (cRPMI, Sigma Aldrich) supplemented with 10% fetal bovine serum (FBS, Sigma Aldrich), 1× penicillin-streptomycin (Pen-Strep, Sigma Aldrich), and L-glutamine (Sigma Aldrich), followed by centrifugation at 300 × g for 5 min. Splenocytes were resuspended in 5 mL of cRPMI, filtered through 40 μm strainers (BD Biosciences, San Jose, CA) and counted using Article Title: Develop a durable, memory-driven, CspZ-targeting Lyme disease vaccine by rationale adjuvant selection Article Snippet: Spleens were rinsed and homogenized in sterile PBS buffer, using a gentleMACS Dissociator (Miltenyi Biotech, Charlestown, MA) and treated with ACK lysis buffer (Lonza, Portsmouth, NH) to remove red blood cells. .. The suspension was then diluted 5-fold with complete Roswell Park Memorial Institute (RPMI) 1640 medium (cRPMI, Sigma Aldrich) supplemented with 10% fetal bovine serum (FBS, Sigma Aldrich), 1× penicillin-streptomycin (Pen-Strep, Sigma Aldrich), and L-glutamine (Sigma Aldrich), followed by centrifugation at 300 × g for 5 min. Splenocytes were resuspended in 5 mL of cRPMI, filtered through 40 μm strainers (BD Biosciences, San Jose, CA) and counted using Centrifugation:Article Title: Developing a durable, memory-driven, CspZ-targeting Lyme disease vaccine by rationale adjuvant selection. Article Snippet: Spleens were rinsed and homogenized in sterile PBS buffer, using a AR TI CL E IN P RE SS gentleMACS Dissociator (Miltenyi Biotech, Charlestown, MA) and treated with ACK lysis buffer (Lonza, Portsmouth, NH) to remove red blood cells. .. The suspension was then diluted 5-fold with complete Roswell Park Memorial Institute (RPMI) 1640 medium (cRPMI, Sigma Aldrich) supplemented with 10% fetal bovine serum (FBS, Sigma Aldrich), 1× penicillin-streptomycin (Pen-Strep, Sigma Aldrich), and L-glutamine (Sigma Aldrich), followed by centrifugation at 300 × g for 5 min. Splenocytes were resuspended in 5 mL of cRPMI, filtered through 40 μm strainers (BD Biosciences, San Jose, CA) and counted using Article Title: Develop a durable, memory-driven, CspZ-targeting Lyme disease vaccine by rationale adjuvant selection Article Snippet: Spleens were rinsed and homogenized in sterile PBS buffer, using a gentleMACS Dissociator (Miltenyi Biotech, Charlestown, MA) and treated with ACK lysis buffer (Lonza, Portsmouth, NH) to remove red blood cells. .. The suspension was then diluted 5-fold with complete Roswell Park Memorial Institute (RPMI) 1640 medium (cRPMI, Sigma Aldrich) supplemented with 10% fetal bovine serum (FBS, Sigma Aldrich), 1× penicillin-streptomycin (Pen-Strep, Sigma Aldrich), and L-glutamine (Sigma Aldrich), followed by centrifugation at 300 × g for 5 min. Splenocytes were resuspended in 5 mL of cRPMI, filtered through 40 μm strainers (BD Biosciences, San Jose, CA) and counted using Staining:Article Title: Developing a durable, memory-driven, CspZ-targeting Lyme disease vaccine by rationale adjuvant selection. Article Snippet: Spleens were rinsed and homogenized in sterile PBS buffer, using a AR TI CL E IN P RE SS gentleMACS Dissociator (Miltenyi Biotech, Charlestown, MA) and treated with ACK lysis buffer (Lonza, Portsmouth, NH) to remove red blood cells. .. The suspension was then diluted 5-fold with complete Roswell Park Memorial Institute (RPMI) 1640 medium (cRPMI, Sigma Aldrich) supplemented with 10% fetal bovine serum (FBS, Sigma Aldrich), 1× penicillin-streptomycin (Pen-Strep, Sigma Aldrich), and L-glutamine (Sigma Aldrich), followed by centrifugation at 300 × g for 5 min. Splenocytes were resuspended in 5 mL of cRPMI, filtered through 40 μm strainers (BD Biosciences, San Jose, CA) and counted using Article Title: Develop a durable, memory-driven, CspZ-targeting Lyme disease vaccine by rationale adjuvant selection Article Snippet: Spleens were rinsed and homogenized in sterile PBS buffer, using a gentleMACS Dissociator (Miltenyi Biotech, Charlestown, MA) and treated with ACK lysis buffer (Lonza, Portsmouth, NH) to remove red blood cells. .. The suspension was then diluted 5-fold with complete Roswell Park Memorial Institute (RPMI) 1640 medium (cRPMI, Sigma Aldrich) supplemented with 10% fetal bovine serum (FBS, Sigma Aldrich), 1× penicillin-streptomycin (Pen-Strep, Sigma Aldrich), and L-glutamine (Sigma Aldrich), followed by centrifugation at 300 × g for 5 min. Splenocytes were resuspended in 5 mL of cRPMI, filtered through 40 μm strainers (BD Biosciences, San Jose, CA) and counted using |
