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Fisher Scientific acridine orange propidium iodide
Acridine Orange Propidium Iodide, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/acridine+orange+propidium+iodide/acridine+orange/pm42143044-290-67-71
Average 86 stars, based on 1 article reviews
acridine orange propidium iodide - by Bioz Stars, 2026-10
86/100 stars

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Suspension:

Article Title: Developing a durable, memory-driven, CspZ-targeting Lyme disease vaccine by rationale adjuvant selection.
Article Snippet: Spleens were rinsed and homogenized in sterile PBS buffer, using a AR TI CL E IN P RE SS gentleMACS Dissociator (Miltenyi Biotech, Charlestown, MA) and treated with ACK lysis buffer (Lonza, Portsmouth, NH) to remove red blood cells. .. The suspension was then diluted 5-fold with complete Roswell Park Memorial Institute (RPMI) 1640 medium (cRPMI, Sigma Aldrich) supplemented with 10% fetal bovine serum (FBS, Sigma Aldrich), 1× penicillin-streptomycin (Pen-Strep, Sigma Aldrich), and L-glutamine (Sigma Aldrich), followed by centrifugation at 300 × g for 5 min. Splenocytes were resuspended in 5 mL of cRPMI, filtered through 40 μm strainers (BD Biosciences, San Jose, CA) and counted using acridine orange–propidium iodide (AOPI, Fisher Scientific) staining on a Cellometer Auto 2000 (Nexcelom Bioscience, Lawrence, MA). ..

Article Title: Develop a durable, memory-driven, CspZ-targeting Lyme disease vaccine by rationale adjuvant selection
Article Snippet: Spleens were rinsed and homogenized in sterile PBS buffer, using a gentleMACS Dissociator (Miltenyi Biotech, Charlestown, MA) and treated with ACK lysis buffer (Lonza, Portsmouth, NH) to remove red blood cells. .. The suspension was then diluted 5-fold with complete Roswell Park Memorial Institute (RPMI) 1640 medium (cRPMI, Sigma Aldrich) supplemented with 10% fetal bovine serum (FBS, Sigma Aldrich), 1× penicillin-streptomycin (Pen-Strep, Sigma Aldrich), and L-glutamine (Sigma Aldrich), followed by centrifugation at 300 × g for 5 min. Splenocytes were resuspended in 5 mL of cRPMI, filtered through 40 μm strainers (BD Biosciences, San Jose, CA) and counted using acridine orange– propidium iodide (AOPI, Fisher Scientific) staining on a Cellometer Auto 2000 (Nexcelom Bioscience, Lawrence, MA). ..

Centrifugation:

Article Title: Developing a durable, memory-driven, CspZ-targeting Lyme disease vaccine by rationale adjuvant selection.
Article Snippet: Spleens were rinsed and homogenized in sterile PBS buffer, using a AR TI CL E IN P RE SS gentleMACS Dissociator (Miltenyi Biotech, Charlestown, MA) and treated with ACK lysis buffer (Lonza, Portsmouth, NH) to remove red blood cells. .. The suspension was then diluted 5-fold with complete Roswell Park Memorial Institute (RPMI) 1640 medium (cRPMI, Sigma Aldrich) supplemented with 10% fetal bovine serum (FBS, Sigma Aldrich), 1× penicillin-streptomycin (Pen-Strep, Sigma Aldrich), and L-glutamine (Sigma Aldrich), followed by centrifugation at 300 × g for 5 min. Splenocytes were resuspended in 5 mL of cRPMI, filtered through 40 μm strainers (BD Biosciences, San Jose, CA) and counted using acridine orange–propidium iodide (AOPI, Fisher Scientific) staining on a Cellometer Auto 2000 (Nexcelom Bioscience, Lawrence, MA). ..

Article Title: Develop a durable, memory-driven, CspZ-targeting Lyme disease vaccine by rationale adjuvant selection
Article Snippet: Spleens were rinsed and homogenized in sterile PBS buffer, using a gentleMACS Dissociator (Miltenyi Biotech, Charlestown, MA) and treated with ACK lysis buffer (Lonza, Portsmouth, NH) to remove red blood cells. .. The suspension was then diluted 5-fold with complete Roswell Park Memorial Institute (RPMI) 1640 medium (cRPMI, Sigma Aldrich) supplemented with 10% fetal bovine serum (FBS, Sigma Aldrich), 1× penicillin-streptomycin (Pen-Strep, Sigma Aldrich), and L-glutamine (Sigma Aldrich), followed by centrifugation at 300 × g for 5 min. Splenocytes were resuspended in 5 mL of cRPMI, filtered through 40 μm strainers (BD Biosciences, San Jose, CA) and counted using acridine orange– propidium iodide (AOPI, Fisher Scientific) staining on a Cellometer Auto 2000 (Nexcelom Bioscience, Lawrence, MA). ..

Staining:

Article Title: Developing a durable, memory-driven, CspZ-targeting Lyme disease vaccine by rationale adjuvant selection.
Article Snippet: Spleens were rinsed and homogenized in sterile PBS buffer, using a AR TI CL E IN P RE SS gentleMACS Dissociator (Miltenyi Biotech, Charlestown, MA) and treated with ACK lysis buffer (Lonza, Portsmouth, NH) to remove red blood cells. .. The suspension was then diluted 5-fold with complete Roswell Park Memorial Institute (RPMI) 1640 medium (cRPMI, Sigma Aldrich) supplemented with 10% fetal bovine serum (FBS, Sigma Aldrich), 1× penicillin-streptomycin (Pen-Strep, Sigma Aldrich), and L-glutamine (Sigma Aldrich), followed by centrifugation at 300 × g for 5 min. Splenocytes were resuspended in 5 mL of cRPMI, filtered through 40 μm strainers (BD Biosciences, San Jose, CA) and counted using acridine orange–propidium iodide (AOPI, Fisher Scientific) staining on a Cellometer Auto 2000 (Nexcelom Bioscience, Lawrence, MA). ..

Article Title: Develop a durable, memory-driven, CspZ-targeting Lyme disease vaccine by rationale adjuvant selection
Article Snippet: Spleens were rinsed and homogenized in sterile PBS buffer, using a gentleMACS Dissociator (Miltenyi Biotech, Charlestown, MA) and treated with ACK lysis buffer (Lonza, Portsmouth, NH) to remove red blood cells. .. The suspension was then diluted 5-fold with complete Roswell Park Memorial Institute (RPMI) 1640 medium (cRPMI, Sigma Aldrich) supplemented with 10% fetal bovine serum (FBS, Sigma Aldrich), 1× penicillin-streptomycin (Pen-Strep, Sigma Aldrich), and L-glutamine (Sigma Aldrich), followed by centrifugation at 300 × g for 5 min. Splenocytes were resuspended in 5 mL of cRPMI, filtered through 40 μm strainers (BD Biosciences, San Jose, CA) and counted using acridine orange– propidium iodide (AOPI, Fisher Scientific) staining on a Cellometer Auto 2000 (Nexcelom Bioscience, Lawrence, MA). ..



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Image Search Results


Co-administration of gemcitabine and BD B10 induced apoptosis pathway in Panc1 cells. Apoptosis was analysed using multiple methods: (a) AO/PI doubling staining was applied to assess morphological changes in Panc1 cells following each treatment, using fluorescence microscopy, n = 3. Images were captured at 100x magnification (scale bar = 100 µm). (b) The proportion of live (green) and dead (red) cells was quantified using ImageJ and normalised to actual cell counts to analyse cell distribution. (c) Diagram of different apoptotic stages: viable cells, early apoptotic cells, late apoptotic cells, and dead/naked nuclei cells. (d) Annexin V-FITC/PI assay was applied to determine the distribution of different stages of apoptotic cells in Panc1 cells using flow cytometry, n = 4. (e) The percentage of each stage of Panc1 cells was statistically quantified by the iQueForecyt software to analyse cell distribution. Data were analysed using GraphPad Prism, with results expressed as mean ± SEM. Comparisons between treatment groups were evaluated using one-way ANOVA to determine statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001).

Journal: Frontiers in Pharmacology

Article Title: Enhancement of gemcitabine toxicity and specificity through PI3K/Akt/Nrf2 pathway inhibition in pancreatic cancer

doi: 10.3389/fphar.2026.1724989

Figure Lengend Snippet: Co-administration of gemcitabine and BD B10 induced apoptosis pathway in Panc1 cells. Apoptosis was analysed using multiple methods: (a) AO/PI doubling staining was applied to assess morphological changes in Panc1 cells following each treatment, using fluorescence microscopy, n = 3. Images were captured at 100x magnification (scale bar = 100 µm). (b) The proportion of live (green) and dead (red) cells was quantified using ImageJ and normalised to actual cell counts to analyse cell distribution. (c) Diagram of different apoptotic stages: viable cells, early apoptotic cells, late apoptotic cells, and dead/naked nuclei cells. (d) Annexin V-FITC/PI assay was applied to determine the distribution of different stages of apoptotic cells in Panc1 cells using flow cytometry, n = 4. (e) The percentage of each stage of Panc1 cells was statistically quantified by the iQueForecyt software to analyse cell distribution. Data were analysed using GraphPad Prism, with results expressed as mean ± SEM. Comparisons between treatment groups were evaluated using one-way ANOVA to determine statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001).

Article Snippet: Subsequently, the cells were stained with a mixture of Acridine orange and propidium iodide (AO/PI) at 10 μg/mL (AO: 300910250, Thermo Fisher Scientific; PI: P4864, Sigma) in the dark for 5 min at 37 °C.

Techniques: Staining, Fluorescence, Microscopy, Flow Cytometry, Software